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Corning Life Sciences 125-ml baffled polycarbonate erlenmeyer flasks
125 Ml Baffled Polycarbonate Erlenmeyer Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/125-ml+erlenmeyer+flasks/125+ml+erlenmeyer+flasks/pm39586758-55-20-23
Average 90 stars, based on 1 article reviews
125-ml baffled polycarbonate erlenmeyer flasks - by Bioz Stars, 2026-09
90/100 stars

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Suspension:

Article Title: Effects of autophagy-inhibiting chemicals on sialylation of Fc-fusion glycoprotein in recombinant CHO cells
Article Snippet: .. Both cell lines were grown in serum-free suspension culture mode in 125-mL Erlenmeyer flasks (Corning, Corning, NY, USA) containing 30 mL of SFM4CHO (Hyclone, Little Chalfont, UK) supplemented with 4 mM glutamine. ..

Article Title: Effects of autophagy-inhibiting chemicals on sialylation of Fc-fusion glycoprotein in recombinant CHO cells.
Article Snippet: .. Both cell lines were grown in serum-free suspension culture mode in 125-mL Erlenmeyer flasks (Corning, Corning, NY, USA) containing 30 mL of SFM4CHO (Hyclone, Little Chalfont, UK) supplemented with 4 mM glutamine. ..

Article Title: Genome-wide CRISPR/Cas9 knockout screening to mitigate cell growth inhibition induced by histone deacetylase inhibitors in recombinant CHO cells.
Article Snippet: Funding information Samsung Research Funding Center of Samsung Electronics, Grant/Award Number: SRFC‐MA1901‐09 Abstract Histone deacetylase inhibitors (iHDACs) have been extensively studied as enhancers of therapeutic protein production in recombinant Chinese hamster ovary (CHO) (rCHO) cell cultures.. However, the addition of iHDACs reduces the viable cell concentration (VCC) in rCHO cell cultures, thereby reducing their potential to enhance therapeutic protein production.. To mitigate the negative effects of iHDACs on VCC, screening using a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9)‐based single‐gene knockout (KO) library in rCHO cells was performed in the presence of CI994, a member of iHDACs, and 10 potential KO genes that enhanced the VCC of CI994‐treated rCHO cells were identified.

Article Title: A precise and sustainable doxycycline-inducible cell line development platform for reliable mammalian cell engineering with gain-of-function mutations.
Article Snippet: The serum-free adapted CS13*-1.0 cell line was cultured in the PowerCHO2CD medium (Lonza) supplemented with 4 mM glutamine (HyClone) and 1 μM MTX (Sigma-Aldrich, St. Louis, MO, USA). .. These suspension cells were grown in 125-mL Erlenmeyer flasks (Corning, Corning, NY, USA) in a humidified 5% CO2 incubator (Memmert GmbH, Schwabach, Germany) at 120 rpm and 37 ◦C and subcultured every three days. .. Viable cell density (VCD) and cell viability were determined with the Countess II FL automated cell counter (Invitrogen, Carlsbad, CA, USA) using the trypan blue dye exclusion method.

Concentration Assay:

Article Title: 4-(2,5-Dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide improves monoclonal antibody production in a Chinese hamster ovary cell culture.
Article Snippet: .. The rCHO cells were inoculated at a target viable cell concentration of 0.3 × 106 cells/mL in 125-mL Erlenmeyer flasks (Corning) with basal medium. ..

Article Title: Development of an in vitro screening system for synthetic signal peptide in mammalian cell-based protein production.
Article Snippet: Optimizing appropriate signal peptides in mammalian cell-based protein production is crucial given that most recombinant proteins produced in mammalian cells are thought to be secreted proteins.. Until now, most studies on signal peptide in mammalian cells have replaced native signal peptides with well-known heterologous signal peptides and bioinformaticsbased signal peptides.. In the present study, we successfully established an in vitro screening system for synthetic signal peptide in CHO cells by combining a degenerate codon-based oligonucleotides library, a site-specific integration system, and a FACS-based antibody detection assay.

Article Title: 4-(2,5-Dimethyl-1 H -pyrrol-1-yl)- N -(2,5-dioxopyrrolidin-1-yl) benzamide improves monoclonal antibody production in a Chinese hamster ovary cell culture
Article Snippet: .. 127 Fed-batch mode culture condition 128 The rCHO cells were inoculated at a target viable cell concentration of 0.3 × 106 cells/mL in 129 125-mL Erlenmeyer flasks (Corning) with basal medium. ..

Article Title: 4-(2,5-Dimethyl-1 H -pyrrol-1-yl)- N -(2,5-dioxopyrrolidin-1-yl) benzamide improves monoclonal antibody production in a Chinese hamster ovary cell culture
Article Snippet: .. The rCHO cells were inoculated at a target viable cell concentration of 0.3 × 10 6 cells/mL in 125-mL Erlenmeyer flasks (Corning) with basal medium. ..



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